Pemanfaatan DNA barcoding untuk ketertelusuran label berbagai produk olahan ikan berbasis surimi komersial

Asadatun Abdullah, Hana Aulia Sativa, Tati Nurhayati, Mala Nurilmala


Surimi-based processed products are prone to mislabeling using raw materials that are not in accordance with food safety requirements. There were cases reporting use of toxic fish tissue in commercial seafood products. This study was aimed to identify and determine the raw materials used in various processed surimi products using cytochrome oxidase I (COI) gene marker. The experiment consisted of DNA isolation, DNA amplification using several target primers namely full-length barcodes, mini-barcodes, as well as specific species primers against poisonous puffer fish Lagocephalus lunaris and genetic analysis. The results of bioinformatics analysis revealed that S1 samples were Coryphaena hippurus or mahi-mahi fish, S2 and S3 samples were Nemipterus bathybius or curated fish and CS samples were Evynnis cardinalis or kuro fish. Detection of samples with species specific primers of puffer fish Lagocephalus lunaris with annealing temperatures of 54°C, 57°C, and 60°C showed no DNA bands in the six samples analyzed.


Abdullah A, Nurilmala M, Sitaresmi KP. 2019. DNA mini-barcodes sebagai penanda molekuler untuk ketertelusuran label pangan berbagai produk ikan layur. Jurnal Pengolahan Hasil Perikanan Indonesia. 22(1):33-40.
Ajitama Y. 2017. Autentikasi kerupuk udang dengan metode DNA barcoding. [skripsi]. Bogor(ID): Institut Pertanian Bogor.
Astuti RT, Darmanto YS, Wijayanti I. 2014. Pengaruh penambahan isolat protein kedelai terhadap karakteristik bakso dari surimi ikan swanggi (Priacanthus tayenus). Jurnal Pengolahan dan Bioteknologi Hasil Perikanan. 3(3):47-54.
Astuti Y. 2018. Deteksi bahan baku ikan pada produk olahan melalui gen penanda COI. [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Barcaccia G, Lucchin M, Cassandro M. 2016. DNA barcoding as a molecular tool to track down mislabeling and food privacy. Diversity. 8(2):1-16.
Bhattacharjee MJ, Laskar BA, Dhar B, Ghosh SK. 2015. Identification and re-evaluation of freshwater catfishes through DNA barcoding. Plos One. 7(11):1-7.
Borah P. 2009. Primer designing for PCR. Science Vision. 11(3):134-136.
Dharmayanti NLP. 2011. Filogenetika molekuler: metode taksonomi organisme berdasarkan sejarah evolusi. Wartazoa. 21(1):1-10
Dieffenbach CW, Lowe TM, Dveksler GS. 1993. General Concept for PCR Primer Design. New York (US) : Cold Spring Harbor Laboratory Press.
Drancourt M, Bollet C, Carlioz A, Martelin R, Gayral JP, Raoult D. 2000. 16s Ribosomal dna sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. Journal of Clinical Microbiology. 38(10): 3623-3630.
Espineira M, Herrero B, Vieites JM, Santaclara FJ. 2010. Validation of end-point and real-time PCR methods for the rapid detection of soy allergen in processed products. Food Additives and Contaminants. 27(4):426-432.
[FAO] Food and Agriculture Organization of the United Nations. 2018. The State of World Fisheries and Aquaculture. New York (US): Pham cu.
Hall BG. 2013. Building phylogenetic trees from molecular data with MEGA. Molecular Biology and Evolution. 30 (5): 1229-1235.
Haye PA, Segovia NI, VeraR, Gallardo MA, Escarate CG. 2012. Authentication of commercialized crab-meat in Chile using DNA barcoding. Food Control. 25: 239-244.
Hillis DM, Bull JJ. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology. 42(2):182-192.
Hsieh CH, Chang WT, Chang CH, Hsieh HS, Chung YL, Hwang DF. 2010. Puffer fish-based commercial fraud identification in a segment of cytochrome b region by PCR–RFLP analysis. Food Chemistry. 121:1305-1311.
Irene. 2013. Penggunaan gen sitokrom β (cyt β) dalam identifikasi spesies anjing, kucing, dan harimau untuk menjamin keaslian produk pangan dan obat. [skripsi]. Institut Pertanian Bogor.
Kalendar R, Lee D, Schulman AH. 2011. Java web tools for PCR in silico PCR, and oligonukleotide assembly and analysis. Genomics. 98:137-144.
Keskin E, Atar HH. 2012. Molecular identification of fish spesies from surimi-based product labeled as Alaskan Pollock. Journal of Applied Ichtiology. (2012):1-4.
Khallaf AG, Ardura A, Borrel YJ, Vazquez EG. 2016. Towards more sustainable surimi? PCR-cloning approach for DNA barcoding reveals the use of species of low trophic level and aquaculture in Asian surimi. Food Control. 61:62-80.
Khosravinia H, Murthy HNN, Parasad DT, Pirany N. 2007. Optimizing factors influecing DNA extracting from fresh whole avian blood. African Journal of Biotechnology. 6(4):481-486.
Kumar S, Stecher G, Tamura K. 2016. MEGA 7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution. 33(7):1870-1874.
Kuske CR, Barns SM, Grow CC, Merill L, Dunbar J. 2006. Environmental survey for four pathogenic bacteria and closely related species using phylogenetic and functional genes. Journal Forensic Science. 51(3):548-558.
Leal MC, Pimentel T, Ricardo F, Rosa R, Calado R. 2015. Seafood traceability: current needs, available tools, and biotechnological challenges for origin certification. Trends in Biotechnology. 30(10): 1-6.
Maulid DY, Nurilmala M. 2015. DNA barcoding untuk autentikasi produk ikan tenggiri (Scomberomorus sp). Jurnal Akuatika. 6(2):154-160.
Muhammad H, Iqbal Z, Iqbal MU, Younas T, Bashir Q. 2016. An efficient method for DNA Isolation from fish fin. Pakistan Journal of Agricultural Science. 53(4): 843-850.
Mulyani Y, Purwanto A, Nurruhwati I. 2011. Perbandingan beberapa metode isolasi DNA untuk deteksi dini koi herpes virus (KHV) pada ikan mas (Cyprinus carpio L.). Jurnal Akuatika. 1(2):1-16.
Muttaqin E, Abdullah A, Nurilmala M, Ichsan M, Simeone BM, Yulianto I, Booth H. 2019. DNA-barcoding as molecular marker for seafood forensics: Species identification of locally consumed shark fish products in the world’s largest shark fishery. IOP Conf. Series:Earth and Environmental Science. 278:1-10.
Noguchi T, Arakawa O.2008. Tetrodotoxin- distribution and accumulation in aquatic organisms, and cases of human intoxication. Marine Drugs. 6:220-242.
Ozturk AR, Can T. 2017. A multiplex primer design algorithm for target amplification of continuous genomic regions. BMC Bioinformatics. 18(306):1-9.
Park JW. 2005. Surimi and Surimi Seafood : Second Edition. Boca Raton [US] : Taylor and Francis Group.
Rostini I. 2013. Pemanfaatan daging limbah filet ikan kakap merah sebagai bahan baku surimi untuk produk perikanan. Jurnal Akuatika. 4(2):141-148.
Sambrook J, Russel DW. 2001. Molecular Cloning: a Laboratory Manual 3rd Edition. New York (USA): Cold Spring Harbor.
Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America. 74(12):5463-5467.
Shokralla S, Hellberg RS, Handy SM, King I, Hajibabaei M. 2015. A DNA mini-barcoding system for authentication of processed fish product. A Nature Research Journal. 5(15894):1-11.

Sultana S, Ali ME, Hossain MA, Asing, Naquiah N, Zaidul IS. 2017. Universal mini COI barcode for the identification of fish species in processed products. Food Research International. 105:19-28.
Syafaruddin, Randriani E, Santoso TJ. 2011. Efektivitas dan efisiensi teknik isolasi dan purifikasi DNA pada jambu mete.Jurnal Tanaman Industri dan Penyegar. 2(2):151-160.
Ward RD, Zemlak TS, Innes BH, Last PR, Hebert DN. 2005. DNA barcoding Australia’s fish species. Philosophical Transactions of The Royal Society Biological Science. 360:1847-1857.
Wawasto A, Santoso J, Nurilmala M. 2018 Karakteristik surimi basah dan kering dari ikan baronang (Siganus sp.). Jurnal Pengolahan Hasil Perikanan Indonesia.21(2):367-377.
Westermeier R. 2004. Protein Purification Protocols. Germany (DE) : Humana Press


Asadatun Abdullah (Primary Contact)
Hana Aulia Sativa
Tati Nurhayati
Mala Nurilmala
AbdullahA., SativaH. A., NurhayatiT., & NurilmalaM. (2019). Pemanfaatan DNA barcoding untuk ketertelusuran label berbagai produk olahan ikan berbasis surimi komersial. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(3), 508-519.

Article Details