The acute toxicity of male Calina papaya leaves (Carica papaya L.) infusion in female mice

Abstract

Background: Papaya (Carica papaya L) is one of the plants that have the potential as a medicine for various diseases. Objective: This study aimed to test the acute toxicity of male Calina papaya leaf extract in female mice by determining the lethal dose 50 (LD50), examining its effect on the organs of the mice, and calculating the most effective concentration of the extract. Methods: This study used 20 DDY strain mice, randomly divided into four treatment groups: the control group was treated with distilled water and the treatment groups were treated with male Calina papaya leaf infusion with doses of 5, 10, and 15 g/kg body weight (BW) orally. Mortality, clinical symptoms, physiological responses, body weight, and relative organ weights were observed. Results: The LD50 value indicated that administering male Calina papaya leaf infusion to female mice was non-toxic. Giving an infusion of male Calina papaya leaf up to a dose of 15 g/kg BW did not cause pathology-clinical symptoms, macro-anatomy organ changes, or death. The infusion did not show a significant effect on increasing or decreasing body weight. Giving an infusion of male Calina papaya leaves at a dose of 10 g/kg BW was proven to be the most effective in maintaining average body weight gain and was not toxic. Conclusion: Based on the research, giving male Calina papaya leaf infusion up to a dose of 15 g/kg BW is non-toxic and does not affect the organs of the mice, with an effective dose of 10 g/kg BW.