Potency of Eucalyptus Oil and Citronella Oil in Suppressing Virulence Factors of Xanthomonas oryzae pv. oryzae
Potensi Minyak Kayu Putih dan Minyak Serai Dapur dalam Menekan Faktor Virulensi Bakteri Xanthomonas oryzae pv. oryzae
Abstract
Bacterial leaf blight, caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo), is an important disease of rice and can cause yield losses of 10-50%. Efforts to control bacterial leaf blight in rice using eucalyptus oil and citronella oil have not been widely reported. This study aims to evaluate the potential inhibition of eucalyptus oil and citronella oil against X. oryzae pv. oryzae isolate code BaK_2 in vitro, focusing on X. oryzae pv. oryzae virulence factors. The virulence factor assay consisted of biofilm formation, exopolysaccharide (EPS) production and motility. The results showed that eucalyptus oil and citronella oil could inhibit the growth of X. oryzae pv. oryzae in vitro. The minimum inhibitory concentration (MIC) was 15% for eucalyptus oil and 5% for citronella oil. Virulence factor tests showed that eucalyptus oil and citronella oil had no significant effect on biofilm formation, but could reduce EPS formation and limit the movement of X. oryzae pv. oryzae by both swimming motility and motility.
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References
Anonim. 2007. Xanthomonas oryzae. EPPO Bulletin. 37:543–553. DOI: https://doi.org/10.1111/j.1365-2338.2007.01162.x.
Ansari TH, Ahmed M, Ara A, Khan MA, Mian MS, Zahan QS, Tomita M. 2018. Yield loss assessment of rice due to bacterial blight at different resistance levels. Bangladesh Journal Plant Pathology. 34:71–76.
Antar A, Lee MA, Yoo Y, Cho MH, Lee SW. 2020. PXO_RS20535, encoding a novel response regulator, is required for chemotactic motility, biofilm formation, and tolerance to oxidative stress in Xanthomonas oryzae pv. oryzae. Pathogens. 9(11):1–18. DOI: https://doi.org/10.3390/pathogens9110956.
Badan Pusat Statistik. 2023. Luas Panen, Produksi, dan Produktivitas Padi Menurut Provinsi 2020-2022. <https://www.bps.go.id/indicator/53/1498/1/luas-panen-produksi-dan-produktivitas-padi-menurut-provinsi.html>. [diakses 02 Januari 2023].
Chouhan S, Sharma K, Guleria S 2017. Antimicrobial activity of some essential oils – present status and future perspectives. Medicines. 4(3):1–21. DOI: https://doi.org/10.3390/medicines4030058.
Ham Y, Kim TJ. 2018. Nitrogen sources inhibit biofilm formation by Xanthomonas oryzae pv. oryzae. Journal of Microbiology and Biotechnology. 28(12):2071–2078. DOI: https://doi.org/10.4014/jmb.1808.08025.
Hulankova R. 2022. The influence of liquid medium choice in determination of minimum inhibitory concentration of essential oils against pathogenic bacteria. Antibiotics, 11(150):1–9. DOI: https://doi.org/10.3390/antibiotics11020150.
Isman MB. 2000. Plant essential oils for pest and disease management. Crop Protection, 19(8–10):603–608. DOI: https://doi.org/10.1016/S0261-2194(00)00079-X.
Li Y, Yan Y, Deng S, Zhang C, Haq F, Chen T, Li Y, Li S, Yang R, Zou L, Chen G. 2020. The Xanthomonas oryzae pv. oryzae type IV pilus alignment subcomplex protein PilN contributes to regulation of bacterial surface‐associated behaviours and T3SS system. Plant pathology. 69(4):744–755. DOI: https://doi.org/10.1111/ppa.13157.
Maryam, N., E. Shad, M. Razmjooei, R. Safdarianghomsheh, F. Delvigne, and M. Khalesi. 2020. Production of xanthan gum using immobilized Xanthomonas campestris cells: effects of support type. Biochemical Engineering Journal, 157(2020):1–13. DOI: https://doi.org/10.1016/j.bej.2020.107554.
Mishra S, Yang X, Ray S, Fraceto LF, Singh HB. 2020. Antibacterial and biofilm inhibition activity of biofabricated silver nanoparticles against Xanthomonas oryzae pv. oryzae causing blight disease of rice instigates disease suppression. World Journal of Microbiology and Biotechnology. 36(55):1–10. DOI: https://doi.org/10.1007/s11274-020-02826-1.
Misra S, Keer PK, Roy S. 2022. Essentials oils for a healthy environment: a systematic review study in various industries promoting tourism, hospitality sectors. International Journal of Health Sciences. 6(S3):9048–9055. DOI: https://doi.org/10.53730/ijhs.v6nS3.8220.
Mukarram M, Choudhary S, Khan MA, Poltronieri P, Khan MM, Ali J, Kurjak D, Shahid M. 2022. Lemongrass essential oil components with antimicrobial and anticancer activities. Antioxidants. 11(1):1–23. DOI: https://doi.org/10.3390/antiox11010020.
Mulyaningsih S, Sporer F, Reichling J, Wink M. 2011. Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Pharmaceutical Biology. 49(9):893–899. DOI: https://doi.org/10.3109/13880209.2011.553625.
Naveena S, Rai B, Ranjan RK. 2020. Efficacy of antibacterial chemicals and essential oils against Xanthomonas oryzae pv. oryzae under in vitro conditions. International Journal of Current Microbiology and Applied Sciences. Special Issue. 11:2763–2771.
Ou SH. 1985. Rice Diseases. Second Edition. Surrey (UK): Commonwealth Mycological Institute.
Palma V, Gutiérrez MS, Vargas O, Parthasarathy R, Navarrete P. 2022. Methods to evaluate bacterial motility and its role in bacterial–host interactions. Microorganisms. 10(563):1–14. DOI: https://doi.org/10.3390/microorganisms10030563.
Reinke R, Kim SM, Kim BK. 2018. Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding. Molecular Genetics and Genomics. 293(6):1565–1575. DOI: https://doi.org/10.1007/s00438-018-1470-1.
Thepbandit W, Buensanteai N, Thumanu K, Siriwong S, Toan Le T, Athinuwat D. 2021. Salicylic acid elicitor inhibiting xanthomonas oryzae growth, motility, biofilm, polysaccharides production, and biochemical components during pathogenesis on rice. Chiang Mai Journal Science. 48(2):341–353.
Wang B, Wu G, Zhang Y, Qian G, Liu F. 2018. Dissecting the virulence‐related functionality and cellular transcription mechanism of a conserved hypothetical protein in Xanthomonas oryzae pv. oryzae. Molecular plant pathology. 19(8):1859–1872. DOI: https://doi.org/10.1111/mpp.12664.
Wińska K, Mączka W, Łyczko J, Grabarczyk M, Czubaszek A, Szumny A. 2019. Essential oils as antimicrobial agents—myth or real alternative?. Molecules. 24(11):1–21. DOI: https://doi.org/10.3390/molecules24112130.
Wonni I, Ouedraogo SL, Ouedraogo I, Sanogo L. 2016. Antibacterial activity of extracts of three aromatic plants from Burkina Faso against rice pathogen, Xanthomanas oryzae. African Journal of Microbiology Research. 10(20):681–686. DOI: https://doi.org/10.5897/AJMR2016.7999.
Xue D, Tian F, Yang F, Chen H, Yuan X, Yang CH, Chen Y, Wang Q, He C. 2018. Phosphodiesterase EdpX1 promotes Xanthomonas oryzae pv. oryzae virulence, exopolysaccharide production, and biofilm formation. Applied and Environmental Microbiology. 84(22):1–15. DOI: https://doi.org/10.1128/AEM.01717-18.
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