Changes in in Vitro Methane Production and Fatty Acid Profiles in Response to Cakalang Fish Oil Supplementation

  • E. H. B. Sondakh Faculty of Animal Science, Sam Ratulangi University
  • M. R. Waani Faculty of Animal Science, Sam Ratulangi University
  • J. A. D. Kalele Faculty of Animal Science, Sam Ratulangi University
Keywords: cakalang fish oil, fatty acids, in vitro fermentation, methane, gas test


This experiment was conducted to determine the effect of cakalang fish oil addition in ruminant feed on in vitro methane production and fatty acid profiles. This experiment consisted of four treatments which were R0 : feed composing of forage and concentrate at a ratio of 60% : 40% without cakalang fish oil (CFO) addition as control feed; R1: R0 added with CFO at 2.5%; R2: R0 added with CFO at 5%, and R3: R0 added with CFO at 7.5%. Fermentation with rumen fluid was done using the Hohenheim Gas Test (HGT); feeds were incubated at 39 oC for 72 hours. At the end of fermentation, samples were obtained and methane production and fatty acid profiles were determined. The experiment was conducted in completely randomised design with four replications. Data were analysed using analysis of variance and differences among treatment means were analysed using Duncan multiple range test. Results showed that CFO supplementation affected (P<0.05) methane production, protozoa numbers and NH3 concentration; whereas the other parameters, i.e. VFA concentration, pH, and microbial protein were not affected. Some fatty acid profiles were influenced by treatments, such as palmitic, stearic, oleic, linoleic, and linolenic (P<0.05), while others, i.e. lauric and miristic were not affected. It is concluded that the best level of CFO supplementation is 5% as this level reduces methane production and increases unsaturated fatty acids without any negative effects on other variables measured.


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Bhatta, R., M. Saravanan, L. Baruah, K.T. Sampath, & C.S. Prasad. 2013. Effect of plant secondary compounds on in vitro methane, ammonia production and ruminal protozoa population. J App. Microbiol. 115: 455-465.

Chaney, A.L., & E.P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130-132.

Cieslak, A., R. Miltko, G. Belzecki, & E. Kwiatkowska. 2006. Effect of vegestable oils on the methane concentration and population density of the rumen ciliate, Eremoplastron dilobum, grown in vitro. J. Anim. Feed Sci. 15: 15-18.

Diaz, A., M. Avendano, & A. Escobar. 1993. Evaluation of sapindus saponaria as a defaunating agent and its effects on different ruminal digestion parameters. Livest. Res. Rural Dev. 5: 1-6.

Dohme, F., A. Machmuller, B.L. Esterman, P. Pfister, A. Wasserfallen, & M. Kreuzer. 1999. The rule of the rumen protozoa for methane suppression caused by coconut oil. Lett. App. Microbiol. 29:87-192.

Doreau, M., B. Salem, & R. Krezminski. 1993. Effect of rapeseed oil supply on in vitro ruminal digestion in cows: comparison of hay and maize silage diets. Anim. Feed Sci. Technol. 44:181-189.

Gao, J., M.Z. Wang, Y.J. Jing, X.Z. Sun, T.Y. Wu, & L.F. Shi. 2016. Impacts of the unsaturation degree of long-chain fatty acids on the volatile fatty acid profiles of rumen microbial fermentation in goats in vitro. J Integrative Agric. 15: 2827–2833.

Harfiah. 2006. Perbandingan daya cerna in vitro bahan kering rumput gajah dan hasil fermentasi campuran rumput lapangan dengan isi rumen. J. Sci. Ethiol. 6: 67-70

Harwanto., L.M. Yusiati, & R. Utomo. 2014. Pengaruh kayu manis (Cinnamomumburmanni Ness ex BI.) sebagai sumber sinamaldehid terhadap parameter fermentasi dan aktivitas mikrobia rumen secara in vitro. Buletin Peternakan 38:71-77

Hristov, A.N., M. Ivan, & T. McAllister. 2004. In vitro effects on individual fatty acids on protozoal numbers and on fermentation products in ruminal fluid from cattle fed a high concentrate, barley-based diet. J. Anim. Sci. 82:2693-2704.

Hungate, R.E. 1975. The rumen microbial ecosystem. Anim. Rev. Ecology Systematics 6:39-66.

Kamra, D.N. 2005. Rumen microbial ecosystem. Special edition: Microbial Diversity. Current Sci. 89:124-135.

Keidane, D., & E. Birǵele. 2003. The efficacy of feed on the intra abomasal pH dynamics in goats. Veterinarija IR Zootechnica 22:58-61

Lopez, P., M.L. Kung Jr., & J.M. Odom. 1996. In vitro of microbial methane production by 9,10-anthraquinone. Anim. Feed Sci. Technol 71: 117-130

Machmuller, A. 2006. Medium-chain fatty acids and their potensial to reduce methanogenesis in domestic ruminants. Agr. Ecosyst. Environ. 112:107-114.

McDonald, P., P.A. Edwards, & J.F.D. Greenhalg. 1988. Animal Nutrition. 4th ed. Longman Sci. and Tech. New York.

Menke, K.H., & H. Steingass. 1988. Estimation of energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Develop. 28: 7-55

Morgavi, D.P., E. Forano, C. Martin, & C.J. Newbold. 2010. Microbial ecosystem and methanogenesis in ruminants. Animal 4: 1024–1036.

Orskov, E.R. 1992. Protein Nutrition in Ruminant. Academic Press Limited. London

Owen, F.N., & R. Zinn. 1988. Protein metabolism of Ruminant animals. In: D.C. 314 Church (Ed), The Ruminant animal Digestive physiology and Nutrition. Reston 315 Book Prentice Hall, Englewood Cliffs, New Jersey.

Plummer, D.T. 1987. An Introduction to Practical Biochemistry. 3rd ed. McGraw-Hill Book Company. London.

Sitoresmi, P.D., L.M. Yusiati, & H. Hartadi. 2009. Pengaruh penambahan minyak kelapa, minyak biji matahari, dan minyak kelapa sawit terhadap penurunan produksi metan di dalam rumen secara in vitro. Buletin Peternakan 33: 96-105.

Sondakh, E.H.B., L. M. Yusiati, H. Hartadi, & E. Suryanto. 2012. The effect of methanogenic inhibitor feed on propionic acid and lamb meat chemical quality. J. Indonesian Trop. Anim. Agric. 37: 183-188.

Sondakh, E.H.B., J.A. Rorong, & J.A.D. Kalele. 2015. Methane gas reduction using virgin coconut oil supplementation in rumen fermentation through in vitro. J. Anim. Prod. 17:144-148.

Steel, R.G.D., & J.H. Torrie. 1980. Principles and Procedures of Statistics. McGraw-Hill Book Co. Inc. New York.

Sung, H.G., Y. Kobayashi, J. Chang, A. Ha, I.H. Hwang, & J.K. Ha. 2007. Low ruminal pH reduces dietary fiber digestion via reduced microbial attachment. J. Anim. Sci. 20: 200-207.

Varadyova, Z., S. Kišidayova, P. Siroka, & D. Jalč. 2007. Fatty acid profiles of rumen fluid from sheep fed diets supplemented with various oils and effect on the rumen ciliate population. Czech J. Anim. Sci. 52: 399–406.

Wasowska, I., M.R.G. Maia, K.M. Niedźwiedzka, M. Czauderna, J.M.C. Ramalho Ribeiro, & E. Devillard. 2006. Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. Br. J. Nutr. 95:1199–1211.