Characteristics of Sie Reuboh Supplemented with Different Combinations of Palm Vinegar (Arenga pinnata) and Kaffir Lime Leaves (Citrus hystrix)

  • Masyitah Masyitah Study Program of Animal Production and Technology, Graduate School, Bogor Agricultural University
  • I. I. Arief Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
  • T. Suryati Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
Keywords: sie reuboh, palm vinegar, kaffir lime leaves, storage room temperature


This research was aimed to analyze the characteristics of sie reuboh processed with the addition of palm vinegar and kaffir lime leaves with different concentrations to produce sie reuboh with a good quality and to maintain the stability of its quality during storage at room temperature. The research consisted of 2 stages. In the first stage, 4000 g of sie reuboh was treated with different doses of palm vinegar and kaffir lime leaves (0 mL and 0 g, 100 mL and 10 g, 120 mL and 20 g, and 140 mL and 30 g) for microbiological analysis.  Sie reuboh with the best result of microbiological analysis was later used in Stage 2 to be stored at room temperature and was analyzed for its physical antioxidant activity, thiobarbituric acid reactive substance (TBARS), and microbiological analysis. The result showed that different levels of palm vinegar and kaffir lime leaves did not significantly affect the aw, pH, yield, and microbiological characteristics of the sie reuboh. Different ratios of palm vinegar and kaffir lime leaves significantly affected (P<0.05) the tenderness of sie reuboh.  Sie reuboh without addition of palm vinegar and kaffir lime leaves showed a better tenderness than sie reuboh added with palm vinegar and kaffir lime leaves. The best treatment based on microbiological analysis was shown by treatment of sie reuboh with 120 mL of palm vinegar and 20 g of kaffir lime leaves and this sie reuboh was later used to test the stability of the sie reuboh when it was stored at room temperature. The results showed that sie reuboh produced by addition of 120 mL of palm vinegar and 20 g of kaffir lime leaves demonstrated antioxidant activity for inhibition of free radicals as was indicated by the stability of TBARS value during 9 days of storage at room temperature. This combination of treatment could also effectively stabilize the quality of sie reuboh, as indicated by a proper storability at maximum limit of total plate count and mold growth until 3 days of storage at room temperature.


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Adam, M.R. & M.O. Moss. 2008. Food Microbiology. 3rd Edition. RSC Pub., Cambridge (UK).

Al-Qadiri, H. M., N. I. Al-Alami, M. Lin., M., Al-Holy, A. G. Cavinato, & B. A Rasco. 2008. Studying of the bacterial growth phases using fourier transform infrared spectroscopy and multivariate analysis. J. Rapid Methods Autom. Microbiol. 16: 73–89.

Arief, I.I., B.S.L. Jenie, M. Astawan, K. Fujiyama, & A.B. Witarto. 2015. Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef. Asian J. Anim. Sci. 9:25–36.

[BAM] Bacteriological Analytical Manual online. 2001. [February 16, 2017].

[BSN] Badan Standar Nasional. 2009. Standar Nasional Indonesia (SNI) No. 08.3 73882009 tentang Batas Maksimum Cemaran Mikroba dalam Pangan, Jakarta.

Birk, T., A.C. Gronlund, B.B. Christensen, S. Knochel, K. Lohse, & H. Rosenquist. 2010. Effect of organic acids and marination ingredients on the survival of Campylobacter jejuni on meat. J. Food Protect. 73: 258-265.

Campo, M. M., G. R. Nute, S. I. Hughes, M. Enser, J. D. Wood, & R. I. Richardson. 2006. Flavour perception of oxidation in beef. J Meat Science 72:303–311. http://dx.doi.10.1016/j.meatsci.2005.07.015.

Cheng, Q & D. Sun. 2008. Factors affecting the water holding capacity of red meat products: a review of recent research advances. Crit. Rev. Food Sci. Nutr. 48:137–159.

Devatkal, S. K. & B. M. Naveena. 2010. Effect of salt, kinnow and pomegranate fruit by product powders on color and oxidative stability of raw ground goat meat 358 during refrigerated storage. Meat Sci. 85: 306–311. 359 http://dx.doi.10.1016/j.meatsci.2010.01.019.

Duarte, R. V., S. A. Moreira, P. A. R. Fernandes, L. G. Fidalgo, M. D. Santos, R. P. Queirós, & J. A. Saraiva. 2014. Preservation under pressure (hyperbaric storage) at 25 °C, 30 °C and 37 °C of a highly perishable dairy food and comparison with refrigeration. CyTA-Journal of Food 13:321–328.

Estiasih, T. & K. G. D. Ahmadi. 2009. Teknologi Pengolahan Pangan. Cetakan Pertama. Penerbit Bumi Aksara, Jakarta.

Hardesty, S. D. 2008. The growing role of local food markets. Am. J. Agric. Econ. 90: 1289-1295.

Li, Z., S. Henning, M. Y. Zhang, A. Zerlin, L. Li, K. Gao, R. P. Lee, H. Karp, G. Thames, S. Bowerman, & H. Heber. 2010. Antioxidant-rich spice added to hamburger meat during cooking results in reduced meat, plasma, and urine malondialdehyde concentrations. J Clin. Nutr. 91: 1180–1184.

Licina, B. Z., O. D. Stefanovic, S. M. Vasic, I. D. Radojevic, M. S. Dekic, & L. R. Comic. 2013. Biological activities of the extracts from wild growing Origanum vulgarel. J. Food Control. 33: 498-504.

Mani-López, E., H. S. García, & A. López-Malo. 2012. Organic acids as antimicrobial to control salmonella in meat and poultry products. Food Res. Int. 45: 713-721. http://dx.doi:10.1016/j.foodres.2011.04.043.

Madduluri, S., K.B. Rao, & B. Sitaram. 2013. In vitro evaluation of antibacterial activity of five indigenous plants extract against five bacterial pathogens of human. Int. J. Pharm. Pharm. Sci. 5:679-684.

Oliveira, C.M., A.C.S. Ferreira, V.D. Freitas, & A.M.S. Silva. 2011. Oxidation mechanisms occurring in wines. Food Res. Int. 44: 1115–1126.

Pereira, A. L. F., T. F. Vidal., M. C., Teixeira, P. F. Oliveir, R. C. F. Pompeu, M. M. Vieira, & J. F. F. Zapata. 2009. Antioksidant effect of mango seed extract and butylated hydroxytoluene in bologna-type mortadella during storage. Food Science and Technology (Campinas). 31: 135-140.

Rahmi, U., M. Yunazar, & S. Adlis. 2013. Profil fitokimia metabolit sekunder dan uji aktivitas antioksidan tanaman jeruk purut (Citrus histrix DC) dan jeruk bali (Citrus maxima Merr). J Kimia Unand. 2:109-114.

Schaechter, M. 2015. A brief history of bacterial growth physiology. Front. Microbiol. (6): article 289.

Sorensen, G. & S. S. Jorgensen. 1996. A critical examination of some experimental variables in the 2-thiobarbituric acid (TBA) test for lipid oxidation in meat products. Zeitschrift fur Lebensmittel Untersuchung und Forschung. 202: 205–210.

Tangkanakul, P., P. Auttaviboonkul, B. Niyomwit, N. Lowvitoon, P. Charoenthamawat, & G. Trakoontivakorn. 2009. Antioxidant capacity, total phenolic content and nutritional composition of Asian foods after thermal trocessing. International Food Research Journal. 16: 571-580.

Theron, M. M. & J. F. R. Lues. 2007. Organic acids and meat preservation: A review. Food Reviews International. 23: 141–158. http://dx.doi10.1080/87559120701224964.

Widaningrum, Miskiyah, & Juniawati. 2015. Efikasi cuka kulit pisang dan air kelapa sebgai penghambat Listeria monocytogenes pada daging ayam. J. Penelitian Pascapanen Pertanian. 12: 43–54.

Yuliani, R., P. Indrayudha, & S. S. Rahmi. 2011. Antibacterial activity of volatil oil of small aromatic lemon leves (Citrus hystrix) against Staphylococcus aureus and Escherichia coli. Phaermacon 12: 50-54.

Zhang, W., S. Xiao, H. Samaraweera, E. J. Lee, & D. U. Ahn. 2010. Improving functional value of meat products. Meat Sci. 86: 15–31. http://dx.doi:10.1016/j.meatsci.2010.04.018

Zhang, L., D. Sun, & Z. Zhang. 2017. Methods for measuring water activity (aw) of foods and its applications to moisture sorption isotherm studies. Crit. Rev. Food Sci. Nutr. 57: 1052–1058.