DETERMINASI STRUKTUR STOK IKAN KEMBUNG LELAKI MENGGUNAKAN METODE PCR-RFLP DI WPP-NRI 711, 572, DAN 573

Abstract

The Indian mackerel (Rastrelliger kanagurta) has important economic value for fishers. High exploitation pressure affects the existence of stocks and balance of the fish in nature. The effectiveness of fisheries management needs to be done, namely by studying a stock structure. A study on the stock structure with genetic analysis can show the genetic status of the stock and gene flow in each stock in the population. This information can be used for the basis of fisheries resource management. The study aims to analyze diversity and genetic structure, as well as stock structure of Indian mackerel in IFMA 711, 572, and 573 using a molecular approach. DNA isolation and extraction yielded 20 total DNA per site (six locations), and it was amplified by PCR method. There were 109 of 120 samples of PCR result which continued to the RFLP stage. The results of the single band cutting process by restriction enzyme (RFLP) were identified using the Popgene32 program. Enzymes that can show polymorphism are AluI and HaeIII. The Indian mackerel stocks from IFMA 711, 572, and 573 are genetically close, indicating that they are a single stock unit. However, genetic distance visualized by dendrogram shows the stock forms two separate clades. It is more effective for statistical purposes to have a separate management unit.