Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines

  • Omat Rachmat Hasbullah Faculty of Medicine, University of Sultan Ageng Tirtayasa, Serang, Banten 42124, Indonesia
  • Ilma Fiddiyanti Faculty of Medicine, University of Jenderal Achmad Yani, Cimahi 40531, Indonesia
  • Dewi Ratih Handayani Faculty of Medicine, University of Jenderal Achmad Yani, Cimahi 40531, Indonesia
  • Endang Sutedja Faculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, Indonesia
  • Darmadji Ismono Faculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, Indonesia
  • Nucki Nursjamsi Hidajat Faculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, Indonesia
  • Wahyu Widowati Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia
  • Ervi Afifah Biomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung 40163, Indonesia
  • Hanna Sari Widya Kusuma Biomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung 40163, Indonesia
  • Rizal Rizal Biomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung 40163, Indonesia. Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia
  • Firman Alamsyah Center for Medical Physics and Cancer Research Ctech Labs Edwar Technology, Tangerang 15143, Indonesia
  • Warsito P. Taruno Center for Medical Physics and Cancer Research Ctech Labs Edwar Technology, Tangerang 15143, Indonesia

Abstract

Osteosarcoma is a highly malignant primary tumor on bone that mainly attacks children and young adolescents. Until now, osteosarcoma therapy still combines some high costs and invasive therapy modalities that may give side effects, such as pain and nausea. Our previous studies suggested that non-contact electric field has anti-proliferative effect on breast cancer cells, in vitro and in vivo. In this study, we were interested studying alternating current electric field effects on osteosarcoma cells progression as well as its potential cytotoxic effects. MG-63 human osteosarcoma cells were cultured and treated with 200 kHz for 6 days. Several genes of interest including p53, p21, MDM2, caspase-3, caspase-8, and caspase-9 were analyzed using real-time qPCR method. Apoptotic index was measured using flow-cytometry assay. Apoptosis was observed through p53-independent p21 pathway (p = 0.011). Cells undergoing apoptosis through internal pathways were shown by the increase of caspase-3 (p = 0.015) and caspase-9 (p = 0.001) levels, but not caspase-8 (p = 0.080). This treatment has successfully reduced the number of living osteosarcoma cells by 14.7% (p = 0.000) and increased cell death up to 4.26% (p = 0.055). Apoptotic index was markedly increased to 16% (p = 0.001). 200 kHz non-contact electric field exposure can disrupt osteosarcoma progression through disruption of normal cell cycle via p53-independent p21 pathway and induction of apoptosis.

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Published
2023-03-02
How to Cite
HasbullahO. R., FiddiyantiI., HandayaniD. R., SutedjaE., IsmonoD., HidajatN. N., WidowatiW., AfifahE., KusumaH. S. W., RizalR., AlamsyahF., & TarunoW. P. (2023). Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines. HAYATI Journal of Biosciences, 30(3), 522-531. https://doi.org/10.4308/hjb.30.3.522-531
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Articles