https://jai.ipb.ac.id/index.php/cbj <p><img style="float: left; padding: 0px 8px 8px 0px;" src="/public/site/images/admincbj/cover_homepage1.jpg" alt=""></p> <div style="text-align: justify; line-height: 19px!important; font: normal 16px 'Roboto', sans-serif!important;">Current Biochemistry (CB) (<a href="https://issn.brin.go.id/terbit/detail/1399256510" target="_blank" rel="noopener">e-ISSN : 2355-7931</a> ; <a href="https://issn.brin.go.id/terbit/detail/1398964790" target="_blank" rel="noopener">p-ISSN : 2355-7877</a>)&nbsp;publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.<br><br>Manuscripts should be written and published in Bahasa or English and should be submitted to CB. Authors should refer to the guidline below when preparing their manuscripts. Previously published manuscripts (either in whole or in part) will not be considered.&nbsp;</div> IPB University en-US Current Biochemistry 2355-7877 https://jai.ipb.ac.id/index.php/cbj/article/view/50305 <p>Diabetic patients are susceptible to severe wound conditions due to high blood sugar levels and bacterial infections. This study aims to determine the formulation and effectiveness of the hydrogel plaster combination of laying hen eggshells with button mushrooms in curing diabetic wounds. The research methods consisted of making the hydrogel, coagulation test, and antibacterial tests. Hydrogels were made with 5%, 10%, and 20% eggshell concentrations. The coagulation activity test used rabbit blood, which was added with glucose. Antibacterial test was performed using <em>Staphylococcus aureus</em> (<em>S. aureus</em>) and <em>Escherichia coli </em>(<em>E. coli</em>)<em>.</em> This research shows that the coagulation activity of F2 and F3 formulations significantly differed from the control, with an average blood clotting time of 64.67 minutes and 63.00 minutes. The antibacterial test results produced a clear zone for <em>S. aureus</em> and <em>E. coli</em> with diameters of 10.27 mm and 9.18 mm for F2, 6.90 mm, and 11.66 mm for F3. This research concludes that hydrogel formulation 3, with 20% eggshell and 6.4% button mushroom, had the best blood clotting effect and antibacterial activity.</p> Akbar Rafiqi Alivianisa Nabilatusyifa Oyi Candra Santoso Putri Ikromatul Hasanah Sabrina Firdayani Rahmah Dimas Andrianto Copyright (c) 2024 Akbar Rafiqi, Alivianisa Nabilatusyifa, Oyi Candra Santoso Putri, Ikromatul Hasanah, Sabrina Firdayani Rahmah, Dimas Andrianto https://creativecommons.org/licenses/by-sa/4.0 2024-01-31 2024-01-31 10 2 46 51 10.29244/cb.10.2.1 https://jai.ipb.ac.id/index.php/cbj/article/view/50836 <p>One disease that correlates with blood glucose levels is diabetes mellitus (DM). According to Basic Health Research (RISKESDAS), in Indonesia by 2030 there will be an increase of up to 21.3 million people suffering from diabetes mellitus. However, nowadays most people are afraid of the side effects of using the drugs they consume and are turning to herbal therapy to lower blood glucose levels. Bay leaves have the benefit of treating diabetes mellitus, hypercholesterolemia, hypertension, diarrhea and gastritis. Phytochemical analysis shows that bay leaves contain essential oils, tannins, flavonoids and terpenoids. The aim of the research was to determine the effect of bay leaf boiled water on reducing blood glucose levels. The research was an experimental One Group Pretest Post-test Design, namely the research subjects had their blood glucose levels measured before (pre-test) and after being given bay leaf boiled water (post-test). 41 respondents in Paok Motong Barat village, Masbagik District, East Lombok Regency had their Blood Glucose Levels (GDS) measured before (pre-test) and after (post-test) giving 300 mL of bay leaf boiled water. Paired Test Statistical Analysis was used to determine the difference in pretest and posttest blood glucose levels given bay leaf boiled water. The results of the study showed that there was a significant decrease in blood glucose levels (p= 0.001) by 15.22 mg/dL to 179.27 mg/dL before administering bay leaf decoction amounting to 194.49 mg/dL. The conclusion is that there is a significant effect (p=0.001) of giving boiled bay leaves on reducing blood glucose levels. The suggestion for this research is to increase the number of respondents in the research as well as examination variables such as fasting blood glucose</p> Nadi Kurniawan Rozikin I Putu Bayu Agus Saputra Sabariah I Nyoman Bagus Aji Kresnapati Copyright (c) 2024 Aji Kresnapati, Rozikin, I Putu Bayu Agus Saputra, Sabariah, I Nyoman Bagus Aji Kresnapati https://creativecommons.org/licenses/by-sa/4.0 2024-01-31 2024-01-31 10 2 52 61 10.29244/cb.10.2.2 https://jai.ipb.ac.id/index.php/cbj/article/view/50456 <p>Formulasi minuman fungsional ekstrak sirih merah dengan penambahan berbagai ekstrak rempah telah diteliti memiliki aktivitas antioksidan, namun hasil penerimaan konsumen masih rendah pada uji sensori karena rasanya pahit. Teknologi mikroenkpasulasi dan nanoenkapsulasi diketahui dapat melindungi komponen bioaktif dan dapat meningkatkan kualitas sensori pangan. Penelitian ini bertujuan untuk mengembangkan minuman fungsional berdasarkan ekstrak daun sirih merah menggunakan teknik mikroenkapsulasi dan nanoenkapsulasi dengan membandingkan ukuran partikel, kadar fenol, aktivitas antioksidan dan sensori dengan minuman <em>ready to drink </em>(RTD). Maltodekstrin 10% digunakan sebagai penyalut pada pembuatan minuman mikroenkapsulasi, sedangkan gum arab dan maltodekstrin digunakan untuk penyalut pada minuman nanoenkapsulasi. Ukuran partikel diuji menggunakan metode <em>Particle Size Analyzer </em>(PSA), total fenolik diuji dengan metode Folin- Ciocalteu, aktivitas antioksidan diuji dengan metode DPPH, dan evaluasi sensori diuji dengan tingkat hedonik (kesukaan). Hasilnya menunjukkan bahwa minuman fungsional minuman nanoenkapsulasi memiliki rata-rata ukuran partikel paling kecil yaitu sebesar 107,9 nm. Kandungan fenolik tertinggi pada minuman RTD sebesar (782,30 ± 2,54 mg GAE / g). Aktivitas antioksidan tertinggi terdapat dalam minuman mikroenkapsulasi yaitu (107,54 ± 0,83 μg / mL). Minuman fungsional nanoenkapsulasi memberikan tingkat hedonik (2,73 ± 1,19) yang lebih disukai dibandingkan minuman mikroenkapsulasi dan RTD. Teknik mikroenkapsulasi dapat meningkatkan aktivitas antioksidan dan teknik nanoenkapsulasi dapat menurunkan aktivitas antioksidan.</p> Tiana Fitrilia Mega Safithri Dinar Mutiara Hikmah Copyright (c) 2024 Tiana Fitrilia Tiana, Mega Safithri Mega, Dinar Mutiara Hikmah Dinar https://creativecommons.org/licenses/by-sa/4.0 2024-02-07 2024-02-07 10 2 74 82 10.29244/cb.10.2.4