Identifikasi Bakteri Pelarut Kalium Indigenus Asal Perkebunan Nanas

  • Kusuma Handayani Pusat Riset Tanaman Pangan, Organisasi Riset Pertanian dan Pangan, Badan Riset dan Inovasi Nasional, Cibinong Science Center, Jl. Raya Jakarta-Bogor, Bogor16911
  • Mirawanty Amin Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung, Jl. Sumantri Brojonegoro Street No. 1, Bandar Lampung 35145

Abstract

The use of relatively high doses of inorganic fertilizers to increase yields in plantation-scale pineapple (Ananas comosus) cultivation can lead to new problems, such as being toxic to plants, polluting the environment, and increasing production costs. The utilization of rhizosphere microbes, such as potassium solubilizing bacteria (KSB) is expected can reduce the dose of inorganic K fertilizer application because it can dissolve potassium-fixed Al and Fe soil so that it is available to plants. This study aims to assess and characterize the potential of BPK in pineapple plantation areas. KSB evaluation was carried out based on the Potassium Solubility Index (IKK), besides that, it also carried out the characterization of bacteria that have the potential as CPC isolates. CPC was isolated from planting blocks with low and high production levels in the vegetative (V) and generative (G) phases with 5 replications. As many as 20 isolates from the CPC population on pineapple plantations were successfully isolated in this study. The lowest K solubility index of 0.6 was obtained in isolate RV1 and the highest, namely 1.2 in isolate RG1. Among these isolates, RG1 is Gram-positive and grows optimally at pH 5, temperature 30°C. Based on the results of the phylogenetic analysis, it is known that this potassium solubilizing isolate has the same level of similarity with the bacterium Bacillus subtilis.

 

Keywords: Bacillus, indigenous, potassium, solubilizing index, temperature

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References

Ahmad M, Nadeem SM, Navee M, Zahir ZA. 2016. Potassium-solubilizing bacteria and their application in agriculture, in: Meena VS, Maurya BR, Verma JP, Meena RS. (Eds.), Potassium solubilizing microorganisms for sustainable agriculture. New Delhi (IN): Springer. 293‒313. https://doi.org/10.1007/978-81-322-2776-2_21

Alibasyah, MR. 2016. Perubahan Beberapa Sifat Fisika dan Kimia Ultisol akibat Pemberian Pupuk Kompos dan Kapur Dolomit pada Lahan Berteras. Jurnal Floratek. 11(1): 75‒87.

Amin M, Rakhisi Z, Ahmady AZ. 2015. Isolation and Identification of Bacillus Species From Soil and Evaluation of Their Antibacterial Properties. Avicenna Journal of Clinical Microbiology and Infection 2(1): e23233. https://doi.org/10.1007/978-81-322-2776-2_21

Balai Penelitian Tanah. 2005. Petunjuk Teknis: Analisis kimia tanah, tanaman, air dan pupuk. Bogor (ID) Badan Penelitian dan Pengembangan Pertanian Departemen Pertanian.

Bagyalakshmi B, Ponmurugan P, Balamurugan A. 2017. Potassium solubilization, plant growth promoting substances by potassium solubilizing bacteria (KSB) from southern Indian Tea plantation soil. Biocatalysis and Agricultural Biotechnology 12(2017): 116–124. https://doi.org/10.1016/j.bcab.2017.09.011

Basyuni Z. 2009. Mineral dan Batuan Sumber Unsur Hara P Dan K. Purbalingga (ID): Universitas Jendral Soedirman

Diep CN, Hieu TN. 2013. Phosphate and potassium solubilizing bacteria from weathered materials of denatured rock mountain, Ha Tien, Kiên Giang province, Vietnam. American Journal of Life Sciences 1(3): 88‒92. https://doi.org/10.11648/j.ajls.20130103.12

Dell’mour M, Leonhard J, Eva O, Markus P, Gunda K, Stephan Hann. (2010). Hydrophilic interaction LC combined with electrospray MS for highly sensitive analysis of underivatized amino acids in rhizosphere research. Journal of Separation Science. 33: 911–922 https://doi.org/10.1002/jssc.200900743

Dilfuza E, Giancarlo R, Stephan W, Rafiq I. 2011. Enzyme activities in the rhizosphere of plants. Soil Enzimoloogy 22(1):149‒166. https://doi.org/10.1007/978-3-642-14225-3_8

Gebreslassie HB. 2016. Effect of Potasium Fertilizer on Crop Production. Journal of Natural Sciences Research 6(7): 81‒86. https://iiste.org/Journals/index.php/JNSR/article/vie w/30184.

Khani AG, Enayatizamir N, Masir MN. 2019. Impact of plant growth promoting rhizobacteria on different forms of soil potassium under wheat cultivation. Letters in Applied Microbiology. 68: 514‒521. https://doi.org/10.1111/lam.13132

Kuta FA. Antifungal effects of Calotropis Procera stem bank extract against Trichoplyton gypseun and Epiderinoplyton Flocosum. African Journal of Biotechnology 7(13): 2116–2118.

Meena VS, Maurya BR, Verma JP. 2014. Does a rhizospheric microorganism enhance K+ availability in agricultural soils? Microbiological Research. 169(5): 337‒347. https://doi.org/10.1016/j.micres.2013.09.003.

Prajapati KB, Modi HA. 2012. Isolation and characterization of potassium solubilizing bacteria from ceramic industry soil. Journal of Microbiology. 1(2‒3): 8‒14.

Raji M, Thangavelu M. 2021. Isolation and screening of potassium solubilizing bacteria from saxicolous habitat and their impact on tomato growth in diferent soil types. Archives of Microbiology. 203(2021): 3147–3161. https://doi.org/10.1007/s00203-021-02284-9

Richter D, Neung-HO. Ryan F, Jason J. 2007. The rizhosphere: an ecological perspective: the rhizosphere and soil formation. Elsevier: Academic Press. https://doi.org/10.1016/B978-012088775-0/50010-0

Rusli A. 2016. Perubahan beberapa sifat fisika dan kimia tanah ultisol akibat pemberian pupuk kompos dan kapur dolomite pada lahan berteras. Jurnal Floratek. 11(1): 75‒87.

Saha M, Maurya BR, Meena VS, Bahadur I, Kumar A. 2016. Identification and characterization of potassium solubilizing bacteria (KSB) from Indo-Gangetic Plains of India. Biocatalysis and Agricultural Biotechnology. 7. 202–209. https://doi.org/10.1016/j.bcab.2016.06.007

Sardans J, Penuelas J. 2021. Potassium control of plant functions: ecological and agricultural implications. Plants 10(2): 410. https://doi.org/10.3390/plants10020419

Selian, R Aulia. 2008. Analisa Kadar Unsur Hara Kalium (K) dari Tanah Perkebunan Kelapa Sawit Bengkalis Riau secara Spektrofotometri Serapan Atom (SSA). Medan (ID): USU Press.

Setiadi Y. 2001. Peranan Mikoriza Arbuskula dalam Rehabilitasi Lahan Kritis di Indonesia. Prosiding Seminar Nasional Mikoriza. Asosiasi Mikoriza Indonesia Cabang Jawa Barat, Hal 1–12.

Setiawati TC, Mihardja A. 2009. Identifikasi dan Kuantifikasi Metabolit Bakteri Pelarut Fosfat dan Pengaruhnya terhadap Aktivitas Rhizoctonia solani pada Tanaman Kedelai. Journal of Tropical Soil 13: 233‒240. https://doi.org/10.5400/jts.2008.v13i3.233-240

Setiawati TC, Mutmainnah L. 2016. Solubilization of potassium containing mineral by microorganism from sugarcane rhizosphere. Agriculture and Agricultural Science Procedia. 9(2016): 108‒117. https://doi.org/10.1016/j.aaspro.2016.02.134

Sindhu SS, Parmar P. 2013. Potassium Solubilization by Rhizosphere Bacteria: Influence of Nutritional and Environmental Conditions. Journal of Microbiology Research 3(1): 25‒31.

Sindhu SS, Parmar P, Phour M. 2014. Nutrient cycling: potassium solubilization by microorganisms and improvement of crop growth. In: Geomicrobiology and Biogeochemistry. Berlin (UK): Springer. pp 175–198. https://doi.org/10.1007/978-3-642-41837-2_10

Sukmadewi DKT, Anas I, Widyastuti R, Anwar S, Citraresmini A. 2021. The effectiveness of application of phosphorous and potassium solubilizing multifunctional microbes (Aspergillus costaricaensis and Staphylococcus pasteuri mutants) on maize growth. Journal of Degraded and Mining Lands Management. 8(2): 2681‒2688. https://doi.org/10.15243/jdmlm.2021.082.2681.

Sulaeman, Suparto, Eviati. 2012. Petunjuk Teknis : Analisis Kimia Tanah, Tanaman, Air, dan Pupuk. Bogor (ID): Balai Penelitian Tanah.

Sharon JA, Hathwaik LT, Glenn GM, Imam SH, Lee CC. 2016. Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth. Journal of soil science and plant nutrition 16(2): 5. https://doi.org/10.4067/S071895162016005000043

Pelezar C. 2008. Dasar-Dasar Mikrobiologi. Jakarta (ID): UI Press.

Prajapati KB, Modi HA. 2012. Isolation and Characterization of Potassium Solubilizing Bacteria From Ceramic Industry Soil. Cibtech Journal of Microbiology. 1(2‒3): 8‒14.

Wang J, Li R, Zhang H, Wei G, Li Z. 2020. Beneficial bacteria activate nutrients and promote wheat growth under conditions of reduced fertilizer application. BMC Microbiology 20(38): 1‒12. https://doi.org/10.1186/s12866-020-1708-z

Verma A, Patidar Y, Vaishampayan A. 2016. Isolation and purification of potassium solubilizing bacteria from different regions of India and its effect on crop’s yield. Indian Journal of Microbiology Research 3(4): 483‒488.

Xiao Y, Wang X, Chen W, Huang Q. 2017. Isolation and identification of three potassium-solubilizing bacteria from rape rhizospheric soil and their effects on ryegrass. Geomicrobiology Journal. 1‒8. https://doi.org/10.1080/01490451.2017.1286416

Zeng X, Liu X, Tang J, Hu S, Jiang P, Li W, Xu L (2012) Characterization and potassium-solubilizing ability of Bacillus circulans. Z1-3. Advanced Science Letter 10: 173–176. https://doi.org/10.1166/asl.2012.3726

Published
2023-10-06
How to Cite
HandayaniK., & Mirawanty Amin. (2023). Identifikasi Bakteri Pelarut Kalium Indigenus Asal Perkebunan Nanas. Jurnal Ilmu Pertanian Indonesia, 29(1), 47-53. https://doi.org/10.18343/jipi.29.1.47