Seleksi, Karakterisasi Morfologi, dan Identifikasi Aktinobakteri Penghasil Mananase Asal Hutan Tanah Jambi untuk Produksi Mananoligosakarida

Rahayu Wulan; Rika Indri Astuti; Yaya Rukayadi; Sri Estuningsih; Anja Meryandini

doi:10.18343/jipi.27.2.279

Rahayu Wulan Sekolah Pascasarjana, Program Studi Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680
Rika Indri Astuti Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680
Yaya Rukayadi Departemen Sains Makanan, Fakultas Sains dan Teknologi Makanan, Universiti Putra Malaysia, Serdang- Salangor 43400, Malaysia
Sri Estuningsih Departemen Klinik Reproduksi Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680
Anja Meryandini Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus Darmaga IPB, Bogor 16680; Pusat Penelitian Sumberdaya Hayati & Bioteknologi (PPSHB)-LPPM IPB, Kampus IPB Darmga, Institut Pertanian Bogor, Bogor 16680

DOI: https://doi.org/10.18343/jipi.27.2.279

Abstract

As the world's largest producer of crude palm oil, Indonesia also produces high palm kernel cake (PKC) by-products. PKC has a high mannan content, so it can be used to produce prebiotic mannan-oligosaccharides (MOS). Enzymatic MOS production can be carried out using actinobacterial mannanase's microbiological approach. The HJ45B-1 isolate was the best isolate, with a peak enzyme production of 0,338 U/mL on the 10th day of incubation. The mannanase enzyme was stable in storage at 27°C. MOS production using 1% PKC substrate produced MOS with the best degree of polymerization (2-4) with incubation for 1-3 hours. Morphological characteristics and molecular identification based on the 16S rRNA gene indicated that the HJ45B-1 isolate was Streptomyces spp.

Keywords: actinobacteria, mannanase, mannan-oligosaccharides, palm kernel cake, Streptomyces

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