Pemetaan Marker AFLP untuk Membuat Peta Genetik Bit Gula

Asep Setiawan

Abstract


One hundred eighty two AFLP marker using primer combination of EcoRI/MseI and PstI/MseI were used in this study to create a DNA marker genetic map of Sugar beet. In average each primer combination yielded 15.5 polymorphic band AFLP marker using primer combination of EcoRI/MseI significant yielded more polymorphic band  than PstI/MseI. From this study a high density DNA marker map coverage all nine chromosomes of sugar beet with  totally length of 744 cM Haldane was established.

Key words: AFLP, Genetic map, Restriction enzyme, Polymorphic


Full Text:

PDF


DOI: http://dx.doi.org/10.24831/jai.v29i2.1546

Editorial Office:
Department of Agronomy and Horticulture, Wing 13 Level 4
Faculty of Agriculture, IPB University
Jl Meranti, Kampus IPB Dramaga, Bogor 16680
Phone (0251) 8629351, Fax (0251) 8629353
Email: jurnal.agronomi@yahoo.com

Copyright Notice: 

All publications by Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) [p-ISSN: 2085-2916, e-ISSN: 2337-3652] is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.