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Abstract

Abstract
The application of hermetic storage, which is a type of modified atmosphere, effectively reduced the growth of Aspergillus flavus during storage and therefore limit the aflatoxin production. However, its relatively high cost is
a major obstacle for its adoption by smallholder farmers and traders. The preparation of two layers of HDPE and polypropylene plastic packaging that is expected to limit the aflatoxin production and prevent the mold growth
during storage. The experimental design used in this study was a completely randomized factorial design. The first factor consisted of three levels: plastic bag (J0), multi-layered plastic packaging composed of plastic bag and hermetic GrainPro bag (J1), and multi-layered plastic packaging composed of plastic bag, high density polyethylene (HDPE), and polypropylene bag (J2). Furthermore, the second factor comprised two levels: without inoculation (M0) and with inoculation (M1). The maize grains that had been stored for three months in J1 and J2
packaging at 12-13% moisture levels has been able to maintain material moisture content remains low. In these two packaging, the percentage of aflatoxin infection was around 2% during three months. On the other hand, the maize grains that had been stored in J1 and J2 packaging at 17-18% moisture content showed that the material moisture content remains high during storage. The fungal infection percentage attain 3-17% in both two types of packaging. Storing the maize grains in J2 packaging with high moisture levels during three months at risk of being contaminated by aflatoxin and seed fermentation.

Abstrak
Penyimpanan jagung melalui modifikasi atmosfer yang terbentuk pada kemasan hermetik telah mampu menghambat pertumbuhan cendawan dan produksi aflatoksin. Namun, kemasan hermetik merupakan produk impor dan harganya relatif mahal untuk diterapkan di tingkat petani dan pedagang pengumpul. Penyusunan dua
lapis kemasan berbahan plastik HDPE dan polipropilen diharapkan mampu menekan produksi aflatoksin dan menghambat pertumbuhan cendawan selama penyimpanan. Rancangan percobaan yang digunakan adalah rancangan acak lengkap faktorial. Faktor pertama adalah jenis kemasan dengan 3 taraf, yaitu (J0) karung plastik, (J1) kemasan berlapis yang tersusun dari karung plastik+plastik hermetik GrainPro, dan (J2) kemasan berlapis yang tersusun dari karung plastik+plastik HDPE+plastik polipropilen. Faktor kedua adalah inokulasi sumber inokulum dengan 2 taraf, yaitu (M0) tanpa inokulasi sumber inokulum, dan (M1) dengan inokulasi sumber
inokulum. Jagung pipil yang telah disimpan selama 3 bulan pada kemasan J1 dan J2 pada kadar air rendah (12-13%) telah mampu mempertahankan kadar air bahan tetap rendah. Di kedua jenis kemasan tersebut, persen infeksi cendawan masih sekitar 2% setelah disimpan selama 3 bulan. Sementara itu, penyimpanan jagung pipil pada kemasan J1 dan J2 pada kadar air tinggi (17-18%) menunjukkan bahwa kadar air bahan tetap tinggi selama penyimpanan. Persen infeksi cendawan mencapai 3-17% pada kedua jenis kemasan tersebut. Penyimpanan jagung pipil dengan kemasan J2 pada kadar air tinggi selama 3 bulan beresiko tercemar aflatoksin serta biji mengalami fermentasi.

Keywords

aflatoxin moisture content multilayer packaging

Article Details

Author Biographies

Dede Risanda, Kampus IPB Dramaga, Bogor 16690

Program Studi Teknologi Pascapanen. Departemen Teknik Mesin dan Biosistem, Fakultas
Teknik Pertanian IPB.

Emmy Darmawati, Kampus IPB Dramaga, Bogor 16690

Departemen Teknik Mesin dan Biosistem, Fakultas Teknik Pertanian IPB.

Meity Suradji Sinaga, Kampus IPB Dramaga, Bogor 16690

Departemen Teknik Mesin dan Biosistem, Fakultas Teknik Pertanian IPB.

References

  1. Chakraverty, A. and R.P. Singh. 2001. Postharvest Technology: Cereals, Pulse, Fruit, and Vegetables. USA. Science publisher, inc and
  2. Das, A., J. Angayarkanni, S. Bhattacharya and M. Palaniswamy. 2012. Evaluation of process parameters influencing aflatoxin B1 synthesis from
  3. Aspergillus flavus MTCC 2798 using rice straw under submerged fermentation. Int. J. Pharm. Bio. Sci. 2(2):94-105.
  4. Lane, B. and C. Woloshuk. 2017. Impact of storage environment on the efficacy of hermetic storage bags. J Stored Prod. 72(2017):83-89
  5. Pratiwi, C., W.P. Rahayu, H.N. Lioe, D. Herawati, W. Broto and S. Ambarwati. 2015. The effect of temperature and relative humidity for Aspergillus
  6. flavus BIO 2237 and aflatoxin production on soybeans. Int Food Res. J. 22(1):82-87
  7. Kusumaningrum, H.D., Suliantari, A.D. Toha, S.H. Putra and A.S. Utami. 2010. Contamination of Aspergillus flavus and aflatoxin at distribution chain
  8. of maize based food product and its influencing factors. J. Tek Ind Pangan. 21(2):171-176
  9. Baoua, I.B., L. Amadou, J.D. Lowenberg-DeBoer and L.L. Murdock. 2013. Side by side comparison of GrainPro and PICS bags for postharvest
  10. preservation of cowpea grain in Niger. J Stored Prod. 54(2013):13-16
  11. [ISTA] International Seed Testing Asociation. 2010. International rules for seed testing. Zurich.Switzerland.
  12. Lacey, J., A. Hamer and N. Magan. 1994. Respiration and losses in stored wheat under different environmental conditions. In: Highley E, Wright EJ,
  13. Banks HJ, Shamp BR (Eds), Proc of 6th Int. working Conf. on Stored Production Protection. Vol. II.
  14. Canberra, Australia. CAB International. 1007-1013 [KEMENTAN] Kementerian Pertanian. 2015. Konsumsi jagung per kapita. [internet] [diunduh 2017 April
  15. 20]. Tersedia pada: http://www.pertanian.go.id Persons K, J.M. Raines and J.M. Rodriquez. 2013.
  16. Antagonistic effects of Saccharomyces cerevisiae on the growth of Aspergillus flavus and Aspergillus parasiticus at varying temperatures. J. Mycology.
  17. 4(1):38-43
  18. Ruiqian, L., Y. Qiaan, D. Thanaboripat and P. Thansukon. 2013. Biocontrol of Aspergillus flavus and aflatoxin production. KMITL Sci. Technol. J.
  19. 4(1): 1-9.
  20. Hadioetomo, R.S. 1993. Mikrobiologi Dasar dalam Praktek. Teknik dan Prosedur Dasar dalam Praktikum. Jakarta: Gramedia Pusaka Utama
  21. Wiliams, S.B., D. Baributsa dan C. Woloshuk. 2014. Assesing purdue improved crop storage (PICS) bags to mitigate fungal growth and aflatoxin
  22. contamination. J Stored Prod. 59(2014):190-196
  23. Weinberg, Z.G., Y. Yan, Y. Chen, S. Finkelman, G. Ashbell and S. Navarro. 2008. The effect of moisture level on high-moisture maize (zea mays)
  24. under hermetic storage conditions – in vitro studies. J Stored Prod. 44(2008):136-144 Risanda, et al.